Fig. 5.

RNA sequencing of serial passages of TU-BcX-4IC compared to the original patient tumor. RNA sequencing was performed on TU-BcX-4IC tumors representing serially transplanted tumors in mice (T1, T4), compared to the primary tumor (T0). Data represents changes in genes unique to the human genome following removal of mouse aligned genes. A Top ten signaling pathways upregulated by both T1 and T4 tumors included ECM organization, cell adhesion and positive regulation of cell migration. B Representation of signaling pathways retained in both TU-BcX-4ICT1 and T4 tumors. C Genes involved in ECM organization, positive regulation of cell matrix, and angiogenesis that were upregulated in both TU-BcX-4ICT1 and T4 tumors. Up-regulated genes included those in collagen, integrin, laminin, metalloproteinase and the semaphorin gene families. Data is represented by a heat map for each gene listed, with blue as the lowest value within a gene group and red as the highest value. Each heat map scale for specific genes (T0, T1, T4 passages) are analyzed separately to represent changes in gene expression over serial transplantation. For example, the heat map range for the semaphorin or laminin gene clusters are unique from the other gene clusters, outlined by black boxes in the heat map