Figure 6: NXTAR restoration inhibits prostate cancer proliferation.

A and B, VCaP (A) and 22Rv1 (B) cells were infected with either pBabe vector or NXTAR expressing constructs and were seeded in androgen-deprived condition. Graph represents number of viable cells determined by trypan blue exclusion assay. C and D, VCaP (C) and 22Rv1 (D) cells infected with pBabe vector or NXTAR expressing constructs and were treated with either vehicle (10% DMSO) or enzalutamide for 9 or 5 days respectively, and viable cells were counted. E, 22Rv1 cells infected with pBabe vector or NXTAR expressing constructs, treated with either vehicle (10% DMSO) or abiraterone for 5 days and the viable cells were counted. F, 1.5 million VCaP cells that were infected either with pBabe or NXTAR vectors were implanted subcutaneously per SCID mice, and allowed to grow till they reached an average volume of ~1200mm³ for pBabe mice. G-I, The tumors were excised, photographed (G) and the tumor weights (H) and volume (I) were recorded. (J) Tumor lysates (n=3 per group) were immunoblotted. Densitometry measurement is provided below the AR blot. Data are represented as mean ± SEM. *p ≤0.05; ** p<0.01; ***p<0.001, two-tailed Student’s t-test. NS, not significant.