Extended Data Fig. 6. Leukocyte attraction via the FPR2-EGR1 pathway is direct and independent of resident skin cells – exemplary confocal microscopy pictures.

a, The pictures show the unprocessed images and the two steps of processing: masking of hairs and ROI determination (see methods). Leukocytes are labeled with different dyes: leukocytes from wild-type mice in magenta, from FPR2^−/− mice in green, and from EGR1^−/− mice in blue. Dye-labeled bacteria are in cyan. Central, non-shaded areas represent the analyzed, computed regions of interest (ROIs). Note that computation and analysis was performed in a 3D manner; the pictures only show 2D slices. b, Processed WT mouse picture in higher magnification centered on the injected bacteria. The exemplary pictures shown here are from the same mice as those in Extended Data Fig. 5 showing spleen controls.