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. Author manuscript; available in PMC: 2022 Jun 6.
Published in final edited form as: Nat Microbiol. 2021 Dec 6;7(1):62–72. doi: 10.1038/s41564-021-01012-9

Figure 3. PSMs trigger EGR1 transcription via the MEK/ERK pathway.

Figure 3.

a, Isolated human neutrophils (1 × 107) were stimulated with synthetic PSMα3 (330 nM) diluted from a stock solution in DMSO for 30 min with or without addition of 10 μM of the indicated pathway inhibitors and EGR1 expression relative to a control that received equal amounts of DMSO was determined by qRT-PCR. n=16/group. Statistical analysis is by Kruskal-Wallis test with Dunn’s post-test vs. values obtained with PSMα3. b, Dose-response curve with different amounts of the MEK/ERK pathway inhibitor U0126 under the same conditions. n=3–5. c, Scheme of PSM-triggered signal transduction and tested pathways and their inhibitors (red font). Pathways that were not PSM-induced (not inhibited by the specific inhibitors) are shaded. a,b, Error bars show the mean ± SD.