FIG. 1.

SMRT activates HDAC3 deacetylase activity in vitro. (a) SMRT core complex components were produced by T7-coupled transcription-translation. Myc-HDAC3 alone or mixed together with Gal4-TBL1 and/or Flag-SMRT was immunoprecipitated with anti-Myc agarose beads to isolate HDAC complexes, and the beads were assayed for HDAC activity. Immunoprecipitation of unprogrammed transcription-translation mix was used as a control. (b) Western blot analysis of the complexes in panel a. The upper panel was probed for SMRT (anti-Flag), the middle panel was probed for TBL1 (anti-Gal4), and the lower panel was probed for HDAC3 (anti-Myc). IP, immunoprecipitation.