FIG. 2.

SMRT binds but does not activate HDAC4. (a) Western blot analysis of HDAC complexes formed in the presence or absence of SMRT. Proteins produced by T7-coupled transcription-translation were mixed and immunoprecipitated (IP) with anti-Myc agarose beads. All samples were resoved by SDS-PAGE and subjected to immunoblotting. Flag-SMRT (top) was probed with anti-Flag. Myc-HDAC4 and Myc-HDAC3 (middle and bottom) were probed with anti-Myc. (b) HDAC activity of the complexes in panel a. Note that 50 μl of translated HDAC3 and 100 μl of HDAC4 were used.