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. 2001 Sep;21(18):6091–6101. doi: 10.1128/MCB.21.18.6091-6101.2001

FIG. 3.

FIG. 3

SMRT contains a DAD. (a) Schematic representation of the SMRT corepressor and deletion mutants used to reconstitute HDAC3 activity. Gal4 DBD fusions to SMRT contained SMRT amino acids 1 to 2473 (full-length SMRT), SMRT amino acids 1961 to 2473 (fragment A), SMRT amino 1531 to 1959 (fragment B), SMRT amino acids 1068 to 1530 (fragment C), SMRT amino acids 1 to 1028 (fragment D), SMRT amino acids 1 to 763 (fragment E), SMRT amino acids 305 to 763 (fragment F), and SMRT amino acids 427 to 559 (fragment G). (b) HDAC activities of HDAC3-containing complexes. Myc-HDAC3 was incubated with equal amounts of Gal4-SMRT fusion proteins, immunoprecipitated with anti-Myc, and subjected to HDAC assay. (c) Immunoblot (IB) of anti-Myc agarose immunoprecipitated (IP) complexes. The upper panel was probed for SMRT polypeptides (anti-Gal4); the lower panel was probed for HDAC3 (anti-Myc).