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. 2001 Sep;21(18):6091–6101. doi: 10.1128/MCB.21.18.6091-6101.2001

FIG. 4.

FIG. 4

The SMRT DAD is comprised of SANT1 and its amino-terminal flank. (a) Schematic representation of SMRT amino acids 305 to 763 containing SANT1 and SANT2. Gal4 DBD–SMRT fusions contained amino acids 305 to 763 (F), amino acids 427 to 669 (F1), amino acids 305 to 559 (F2), amino acids 305 to 489 (F3), amino acids 305 to 427 (F4), amino acids 305 to 455 (F5), amino acids 395 to 489 (F6 [DAD]), and amino acids 427 to 489 (F7). (b) HDAC activities of HDAC3-containing complexes. Myc-HDAC3 was incubated with equal amounts of Gal4-SMRT fusions, immunoprecipitated with anti-Myc, and subjected to HDAC assay. (c) Immunoblot (IB) of anti-Myc-precipitated complexes. The upper panel was probed for SMRT polypeptides (anti-Gal4), and the lower panel was probed for HDAC3 (anti-Myc). IP, immunoprecipitation.