FIG. 8.

Purification of an enzymatically active HDAC3-SMRT complex. (a) Coomassie stain (top) of purified HDAC3 complex. Sf9 cells were mock infected (control) or infected with baculovirus expressing Flag-HDAC3, Myc-SMRT amino acids 1 to 587, or both. Sf9 lysates were purified by anti-Flag affinity chromatography and resolved by SDS-PAGE. Molecular weight markers (MW) and major polypeptides are indicated. Identities of complex components were verified by immunoblotting with anti-Myc and Flag-HDAC3 (below the Coomassie stain). (b) HDAC activities of the proteins in panel a.