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. 2001 Sep;21(18):6091–6101. doi: 10.1128/MCB.21.18.6091-6101.2001

FIG. 9.

FIG. 9

The SMRT DAD is required for repression and deacetylase activity. (a) Repression of a Gal4-SV40-luciferase reporter by Gal4-SMRT 1-763 or Gal4-SMRT 1-763Δ DAD. Fold repression is expressed relative to Gal4 DBD. (b) In vitro HDAC activity of Myc-HDAC3 incubated with either Gal4 DBD, Gal4–SMRT 1-763, or Gal4–SMRT 1-763Δ DAD. HDAC3 complexes were immunoprecipitated (IP) with anti-Myc, and HDAC activity was expressed as the amount of acetate released. (c) HDAC activity of Gal4 DBD, Gal4–SMRT 1-763, or Gal4–SMRT 1-763Δ DAD immunoprecipitated by anti-Gal4 agarose after transfection into 293T cells. (d) Immunoblot (IB) of anti-Gal4 immunoprecipitates from 293T cells transfected with Gal4 DBD, Gal4–SMRT 1-763, or Gal4–SMRT 1-763Δ DAD. Inputs (2%) are shown on the left for comparison. The upper panels were probed for endogenous HDAC3 (anti-HDAC3), and the lower panels were probed for Gal4 DBD and Gal4-SMRT polypeptides (anti-Gal4).