FIG. 1.

Differential association of tyrosine-phosphorylated p92 with Clnk and SLP-76 in activated hemopoietic cells. Cells were activated as detailed in the text. After lysis, individual polypeptides were immunoprecipitated with the indicated antibodies (e.g., anti-Clnk [αClnk]), and the presence of associated phosphotyrosine-containing molecules was determined by immunoblotting with antiphosphotyrosine (αP.tyr) antibodies. The migrations of prestained molecular mass markers are indicated on the right, while those of p130, p92, SLP-76, Clnk, LAT, and the heavy chain of Ig [Ig(H)] are shown on the left. (A) IL-2-dependent antigen-specific T-cell line 2.5. The ∼70-kDa tyrosine-phosphorylated product that was nonspecifically coimmunoprecipitated with the various antibodies in activated cells is likely to be Zap-70. Exposure, 16 h. (B) IL-3-dependent myeloid cell line B6SutA₁. Exposure, 16 h.