FIG. 2.
Association of Clnk SH2 domain with tyrosine-phosphorylated p92. The abilities of various GST fusion proteins to associate with tyrosine-phosphorylated molecules from lysates of resting or activated B6SutA1 cells were examined in an in vitro binding assay. Anti-Clnk (αClnk) immunoprecipitates were also performed in parallel in order to reveal the migration of p92. The positions of prestained molecular weight markers are shown on the right; those of p92, Clnk, and heavy chain of Ig [Ig(H)] are indicated on the left. Exposure, 48 h. αP.tyr, antiphosphotyrosine antibody; wt, wild type; NRS, normal rabbit serum.