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. 2021 Dec 24;8(1):e08673. doi: 10.1016/j.heliyon.2021.e08673

Figure 1.

Figure 1

hTERT promotes the epithelial to mesenchymal transition and cell migration in A549 and H1299. A) Immunoblot analysis of E-cadherin, N-cadherin, and vimentin on hTERT knockdown B) Immunofluorescence assay showing knockdown of hTERT in A549 cells increased the expression level of cytokeratin-18a. C, D) hTERT overexpression in A549 leads to an increased expression of mesenchymal marker (N-cadherin and vimentin) and decreased epithelial markers (E-cadherin and cytokeratin-18a). E) hTERT expression plasmid was also transfected in H1299 cells, showing increased mesenchymal protein (vimentin) on hTERT overexpression. F, G) Migration assay in hTERT knockdown and overexpression in lung cancer cells (A549 and H1299), hTERT down-regulation reduced, while overexpression promoted the cell migration (Mean ± SE, n = 3). Full and non-adjusted images of immunoblots were shown in the supplementary material (Figure SM 1).