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. 2021 Dec 24;8(1):e08673. doi: 10.1016/j.heliyon.2021.e08673

Figure 2.

Figure 2

c-MET expression is regulated by hTERT. A) Immunoblot analysis of phospho-c-MET, c-MET proteins after treatment with different doses of hTERT shRNA in A549. hTERT shRNA reduced the c-MET expression in a dose-dependent manner. B) Immunofluorescence of c-MET in hTERT knockdown A549 also shows the positive association between hTERT and c-MET expression. C, D) hTERT overexpression in A549 (left panel) and H1299 (right panel) shows the increased level of c-MET expression. E) Immunofluorescence of the c-MET protein in hTERT overexpressing A549 cells. F) hTERT knockdown A549 cells were starved for 12 h then stimulated by HGF (20 ng/ml) for 24 h. Expression of phospho and total c-MET were analyzed by western blotting. HGF treatment showed no significant promoting effect on the c-MET expression in cells knocked down for hTERT expression. G) Similar to the previous experiment, for pBABE-hTERT transfection, A549 cells were starved and treated with HGF. Western blots showed that hTERT overexpression has a synergistic stimulatory effect with HGF on the expression of c-MET. Full and non-adjusted images of immunoblots were shown in the supplementary material (Figure SM 2)