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. 2021 Dec 24;8(1):e08673. doi: 10.1016/j.heliyon.2021.e08673

Figure 4.

Figure 4

p53 and c-Myc play a critical role in hTERT mediated regulation of c-MET expression. Immunoblot analysis is showing the p53 and c-Myc expression as influenced by (A) hTERT downregulation and (B) over-expression in A549. (C) p53 and c-Myc expression were observed in A549 cells after hTERT knockdown and treatment with HGF. (D) Immunoblot analysis of c-MET, phospho c-MET expression in p53 knockdown A549 cells. (E) Western blot analysis of c-MET and Phospho-c-MET in p53-shRNA transfected A549 cells followed by HGF treatment showed that p53 knockdown increased the c-MET level, and HGF treatment further elevated the expression and phosphorylation of c-MET. (F) Western blot analysis of Phospho-c-MET and c-MET in c-Myc knockdown A549. (G) Western blot analysis of c-MET and Phospho-c-MET in A549 cells knocked down for c-Myc and treated with HGF. c-Myc downregulation reduced the c-MET level, and further HGF treatment in c-Myc knockdown A549 cells does not significantly restore c-MET expression. Full and non-adjusted images of immunoblots were shown in the supplementary material (Figure SM 3)