Fig. 3.

SNHG5 inhibited miR-299 expression while promoted BACH1 expression. A qRT-PCR measured miR-299 and BACH1 levels after silencing of SNHG5. B BACH1 protein expression was detected by western blotting after knockdown of SNHG5. C SNHG5, miR-299 and BACH1 levels were tested by qRT-PCR after transfecting with miR-299 mimics/inhibitor. D BACH1 expression was examined after transfecting with miR-299 mimics/inhibitor using western blotting. E Upper panel, the binding sites of wild-type or mutant SNHG5 and miR-299. Lower panel, the luciferase activity was analyzed by dual-luciferase reporter assay. F Upper panel, potential miR-299 binding sites on the BACH1 3’-UTR. Lower panel, dual-luciferase reporter assay was performed to estimate the luciferase activity. *p < 0.05, **p < 0.01, ***p < 0.001