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. 2021 Aug 5;29(1):65–76. doi: 10.1007/s12282-021-01281-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — MiR-299 inhibited cell growth and glycolysis in breast cancer. BC cells were transfected with miR-299 mimics and miR-299 inhibitor or their negative control. A colony formation assay analyzed cell proliferation. B The cell cycle profiles were detected by flow cytometry. C Glucose consumption and D lactate production were evaluated by kits. E The expression of HK2, PFK1 and GAPDH were measured using western blotting. β-Actin was used as an internal control. *p < 0.05, **p < 0.01, ***p < 0.001