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. 2021 Dec 23;12:782792. doi: 10.3389/fimmu.2021.782792

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Copyright © 2021 Zhu, Zou, Liu, Wang, Ma, Yin, Peng, Li, Yang, Ren, Zhang, Zhou, Wang, Cao and Xu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The expression of HMGB1 in mouse decidual tissue. (A) The expression of HMGB1 in mouse decidual tissue was investigated by immunohistochemistry staining. Scale bar represents 50 μm and 20 μm. (n = 6/group). (B) Quantitative assessment of the HMGB1 positive staining area in decidua using ImageJ software. (C) The protein levels of HMGB1 were identified by Western blot analysis. (D) The quantification data analysis of HMGB1 in the mouse decidual tissues. Data were expressed as mean ± SEM. (n = 6/group). (E) The serum HMGB1 levels of mice among control group, URSA group, and URSA+ASPL group were analyzed by ELISA. All the results were representatives of three independent experiments and data were expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (Chart D n = 11 in control group; n = 14 in URSA group; n = 11 in URSA+ASPL group).