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. 2021 Dec 23;12:764074. doi: 10.3389/fpls.2021.764074

FIGURE 3.

FIGURE 3

GmMYB133 negatively affects hypocotyl elongation in Arabidopsis. (A) Photograph of untransformed (UT) plant and four transgenic Arabidopsis lines overexpressing GmMYB133 (OX-16, OX-8, OX-14, and OX-15). (B) The expression of GmMYB133 in UT and four transgenic Arabidopsis lines. The expression level in the transgenic line OX-16 was set as 1. (C) Photograph of 36-day-old plants of UT and four transgenic Arabidopsis lines. (D,E) Photograph of hypocotyl elongation (D) and hypocotyl length assay (E) of UT and four transgenic lines. Nine-day-old seedlings were used for the hypocotyl length assay. (F) The expression patterns of light-responsive genes and auxin-associated genes in UT and three transgenic Arabidopsis lines (e.g., AtPIF4, AtBBX24, AtIAA19, AtIAA29, AtSAUR1, AtSAUR21, and AtSAUR26). Total RNAs for (B,F) were exacted from 14-day-old transgenic seedlings and UT. Values were normalized against the gene AtACTIN8, and the expression level in UT was set as 1. Error bars in (B,E,F) indicate SE of three biological and technical replicates, and significant differences are denoted by asterisks: **p < 0.01.