Fig. 4.

TRP channels in metastatic EC biopsies: A,  C Heat maps of mRNA expression of epithelial (CDH1, KRT18, EPCAM, MMP7) and mesenchymal (MMP2, MMP9, CTSB, ACTA2) markers and TRP channels (TRPV2, TRPV4, TRPV6, TRPC1, TRPC4, TRPC6, TRPM4 and TRPM7) in primary and metastatic tumor biopsies. N = 22 p.s. Results are shown as mean relative expression to the geometric mean of housekeeping genes HPRT, PGK, ACTB and GAPDH. B,  D Relative expression of marker genes and TRP channels displaying a significant association with either the primary or metastatic nature of the tumour biopsy. *p < 0.05 **p < 0.01, ***p < 0.001 using univariate linear regression with mRNA expression (ΔCT) as dependent variable and biopsy status as independent variable. N = 22 p.s. E Pearson correlation coefficients between TRP channels and epithelial (CDH1, EPCAM, KRT18, MMP7) and mesenchymal (CDH2, ACTA2, MMP2, MMP9, ZEB1 and CTSB) marker genes in metastatic EC biopsies. Red indicates a positive correlation, while blue indicates a negative correlation. *p < 0.05 **p < 0.01. N = 22 p.s