Fig. 9.

TRP channel expression in EC cells: A Heat map of mRNA expression of TRP channels (TRPV2, TRPV4, TRPV6, TRPC1, TRPC4, TRPC6, TRPM4 and TRPM7) in EC cells EM018, EM033, EM046 and EM012. B % of THC responders in EM018 (n = 504), EM033 (n = 678), EM046 (n = 718) and EM012 (n = 707) cells. **p < 0.01; ***p < 0.001 using One-way ANOVA and Tukey’s multiple comparison test. N = 3–5 measurements. Data are represented as mean ± SD. C–F Representative Ca²⁺ traces of THC-induced [Ca²⁺]_i changes in EM018 (C), EM033 (D), EM046 (E) and EM012 (F) cells with each line representing a single cell. Representative colour-coded Fura-2 [Ca²⁺]_I ratio images during measurement of the cells are indicated on the graphs: basal situation (i), after THC application (ii), and after application of ionomycin (iii). Pseudo-colour ratio images were obtained using Nikon software. Scale bar = 100 µm