Fig. 6.

Reduction of acrolein levels in vitro by PLZ and PEH (from Matveychuk 2015, with permission). Acrolein and PLZ or PEH were incubated in phosphate-buffered saline, pH 7.4, for 30 min. Following incubation, 200 µl samples were adjusted to pH 4 and 50 µl of O-(2,3,4,5,6-pentafluorobenzyl)-hydroxylamine HCl was added to give a concentration of 20 mM. After 60 min of incubation at room temperature, the samples were acidified and the derivatized aldehyde was extracted with 300 µl hexane. A portion of the hexane layer was used for GC–MS analysis. Data are normalized relative to controls and represent means ± SEM (N = 5). All values significantly differ from controls