Fig. 8.
ASH2L is implicated in the cis regulation of USP30-AS1 in USP30 and ANKRD13A expression. The interaction between USP30-AS1 and ASH2L is predicted using catRAPID (http://service.tartaglialab.com/update_submission/392876/94a348ba99). A Interaction profile; B interaction matrix. C RNA pull down assay: the full length of USP30-AS1, the antisense of USP30-AS1, as well as the specific regions of the lncRNA were synthesized to pull down ASH2L in HL-60 cells. D ASH2L was knocked down or overexpressed in KG-1 and HL-60 cells, followed by PCR assay. E Expression of USP30 and ANKRD13A was tested by PCR assay in KG-1 and HL-60 cells with USP30-AS1 KD, USP30-AS1 OE, ASH2L KD or ASH2L OE. F We conducted ChIP assay using H3K4me3 as the IP protein in KG-1 and HL-60 cells with USP30-AS1 KD, USP30-AS1 OE, USP30-AS1250–550 OE, ASH2L KD or ASH2L OE. Significant differences are indicated by *p < 0.05, **p < 0.01 and ***p < 0.001, n = 3