Fig. 1.

The treatment of carvacrol (CARV) diminished H₂O₂ and GSK-mediated increase of cCa²⁺ concentration via inhibition of TRPM2 and TRPV4 in the SH-SY5Y cells. (Mean ± STD). The SH-SY5Y cells in the dishes of control (Cntr), H₂O₂, GSK, H₂O₂+CARV, and GSK+CARV groups were stained with the dye of Fluo 3-AM (1 µM for 60 min). After washing the cells, they were stimulated by H₂O₂ (1 mM) or GSK (100 nM), although they were inhibited by CARV (100 µM). The representative images groups on the increase of cCa²⁺ concentration through TRPM2 inhibition in the Cntr, H₂O₂, and H₂O₂+CARV in the confocal microscope with 40x oil objective were shown a, although the representative images on the cCa²⁺ concentration through TRPV4 inhibition in the groups of Cntr, GSK, and GSK+CARV groups in the LSCM-800 microscopee with 40x oil objective were shown d. The line (1b) and column (1c) mean fluorescence intensities of the Fluo 3-AM (150 s) are shown in the groups of Cntr, H₂O₂, and H₂O₂+CARV in the b and c, although the line (1e) and column (1f) mean fluorescence intensities of the Fluo 3-AM (150 s) are shown in the groups of Cntr, GSK, and GSK+CARV are shown in the e and f. The scale bar was kept as 10 μm. One example image of each figure was selected from 25-30 SH-SY5Y of 6 independent experiments for each condition (^ap ≤ 0.05 vs. Cntr. ^bp ≤ 0.05 vs. H₂O₂ or GSK group)