FIG. 1.

Strain Δ70k grows similar to the parental strain. The Δ70k and tim10-1 strains and the parental (wild-type [WT]) strain (Table 1) were grown to mid-log phase at 25°C in liquid YPD. Cultures were serially diluted by a factor of 3 and spotted onto YPD plates. Plates were incubated for 3 days at 25 or 37°C. WT, GA74-1A (10); tim10-1, temperature-sensitive tim10-1 allele integrated into LEU2 locus (15); Δ70k, strain in which 70-kDa Tim9p-Tim10p and Tim8p-Tim13p complexes are not detectable by immunoblot analysis.