FIG. 2.

Abundance of the TIM22 import components is decreased in strain Δ70k. (A) The parental strain (wild type [WT]) and strain Δ70k were grown at 37°C in lactate medium. The mitochondria were isolated, and aliquots corresponding to total micrograms of mitochondrial protein (μg prot) were analyzed by SDS-PAGE and immunoblotting with rabbit antisera monospecific for Tim10. Blots were treated with ¹²⁵I-protein A and subjected to autoradiography. (B) As in panel A, except that equal amounts (50, 100, and 150 μg) of mitochondrial proteins are loaded. Antibodies are listed on the left. AAC, ADP/ATP carrier. (C) As in panel A, except that equal amounts of mitochondrial proteins are loaded.