TABLE 1.
Saccharomyces cerevisiae strains used in this study
| Strain | Genotype | Source or reference | Comments |
|---|---|---|---|
| GA74-1A | MATaade8 his3 leu2 trp1 ura3 | 10 | Parental strain |
| GA74-6A | MATα ade8 his3 leu2 trp1 ura3 | 10 | Parental strain |
| GA74-1A.d | MATa/α ade8/ade8 his3/his3 leu2/leu2 trp1/trp1 ura3/ura3 | 10 | Parental strain |
| CK13 | MATα ade8 his3 trp1 ura3 Δtim10::HIS3 tim10-1:LEU2 | 15 | Used for segregation analysis of second-site suppressor locus |
| CK14 | MATaade8 his3 leu2 ura3 Δtim10::HIS3 tim10-1:TRP1 | 15 | Original mutant used to generate second-site suppressors, designated tim10-1 |
| CK18 | MATaade8 his3 leu2 ura3 Δtim10::HIS3 tim10-1:TRP1 tim9199A→T | 15 | Extragenic suppressor strain, designated tim10-1 Ts+ |
| CK19 | MATa/α ade8/ade8 his3/his3 ura3/ura3 Δtim10::HIS3/Δtim10::HIS3 trp1/tim10-1:TRP1 leu2/tim10-1:LEU2 TIM9/tim9199A→T | 15 | CK18 × CK13 |
| CK22 | MATa/α ade8/ade8 his3/his3 leu2/leu2 trp1/trp1 ura3/ura3 TIM9/Δtim9::HIS3 | 15 | Used to generate CK26; one copy of TIM9 is deleted |
| CK26 | MATaade8 his3 trp1 ura3 leu2 Δtim9::TRP1 [ptim9199A→Ts:URA] | This study | Tim9p is replaced by Tim9S67Cp, designated Tim9S67C |
| CK73 | MATa/α ade8/ade8 his3/his3 ura3/ura3 Δtim10::HIS3/Δtim10::HIS3 leu2/tim10-1:LEU2 trp1/tim10-1 ts:TRP1 TIM8/Δtim8::URA3 TIM9/tim9199A→T | This study | CK19 in which one copy of TIM8 was disrupted with URA3 |
| CK79 | MATaade8 his3 trp1 ura3 Δtim10::HIS3 tim10-1ts:LEU2 Δtim8::URA3 tim9199A→T | This study | Spore from CK73, designated Δ70k |
| CK80 | MATaade8 his3 leu2 ura3 Δtim10::HIS3 tim10-1 ts:TRP1 Δtim8::URA3 tim9199A→T | This study | Spore from CK73 |