Figure 4.
STING activation promotes FADS2-dependent desaturation
(A) Ifnβ and Cxcl10 mRNA levels in 293T cells transfected or not with dsDNA for 24 h (n = 3).
(B) WCEs of cells treated as in (A) were analyzed by WB using indicated antibodies.
(C) Sum of indicated PUFAs, LC-PUFAs, and derivatives in samples prepared as in (A).
(D) Ratio between total omega-6 and omega-3 PUFAs and derivatives in samples from (C).
(E) Ifnβ and Cxcl10 mRNA levels in WT and Trex1-deficient MEF cells (n = 3).
(F) WCEs of WT and Trex1-deficient MEF cells were analyzed by WB using indicated antibodies.
(G) Sum of indicated PUFAs, LC-PUFAs, and derivatives in WT and Trex1-deficient MEF cells.
(H) Ratio between total omega-6 and omega-3 PUFAs and derivatives in samples from (G).
All graphs present means ± SEM. p values were determined by Student’s t test. ns, not significant; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.