REAGENT/ RESOURCE	SOURCE	IDENTIFIER
Antibodies
HA	BioLegend	Cat# 901501; RRID:AB_2565006
Flag M2	Sigma Aldrich	#F1804-200UG; RRID:AB_262044
HSP90	Cell Signaling	#C45G5; RRID:AB_2233307
GAPDH	Proteintech	60004-1-Ig; RRID:AB_2107436
FADS2	Thermofischer	#PA587765; RRID:AB_2804393
FADS1	Proteintech	10627-1-AP; RRID:AB_2231403
Calnexin	Millipore	#AB2301; RRID:AB_10948000
pIRF3	Cell Signaling	#4947 (4D4G); RRID:AB_823547
IRF3	Cell Signaling	#11904 (D6I4C); RRID:AB_2722521
IRF3	Cell Signaling	#4302 (D83B9); RRID:AB_1904036
pTBK1	Cell Signaling	#5483 (D52C2); RRID:AB_10693472
TBK1	Cell Signaling	#3504 (D1B4); RRID:AB_2255663
pSTING	Cell Signaling	#72971 (D8F4W); RRID:AB_2799831
STING	Cell Signaling	#13647 (D2P2F); RRID:AB_2732796
TREX-1	Santa Cruz Biotechnology	sc133112; RRID:AB_2208802
cGAS	Cell Signaling	#31659 (D3080); RRID:AB_2799008
UCP1	Sigma	U6382; RRID:AB_261838
GFP	Abcam	ab290; RRID: AB_303395
IFNAR	Abcam	ab124764; RRID:AB_10972855
Horse anti-mouse IgG	Cell Signaling	#7076; RRID:AB_330924
Mouse Anti-rabbit IgG	Cell Signaling	#7074; RRID:AB_2099233
Mouse Anti-rabbit IgG (Conformation Specific)	Cell Signaling	#3678; RRID:AB_1549606
Goat anti-Rabbit IgG (H+L), Alexa Fluor 488	Thermoficher	#R37116; RRID:AB_2556544
Goat anti-Mouse IgG (H+L) Alexa Fluor 594	Thermoficher	#R37121; RRID:AB_2556549
Donkey anti-human IgG coupled to AlexaFluor 647	Invitrogen	#A-21447; RRID:AB_141844
Bacterial and virus strains
HSV KOS-64-GFP strain	Gift from Soren.R. Paludan	N/A
Alpha (B.1.1.7) variant of SARS-CoV-2	Olivier Schwartz	N/A
Beta (B.1.351) variant of SARS-CoV-2	Olivier Schwartz	N/A
Chemicals, peptides, and recombinant proteins
DMEM	Lonza	BE12-614F
RPMI 1640	GIBCO	21875034
OPTIMEM	GIBCO	51985
FBS	Eurobio	CVFSVF00-01
Human serum	Sigma	H4522
L-Glutamine	GIBCO	A2916801
Penicillin-streptomycin	GIBCO	10378016
0.25% trypsin EDTA	GIBCO	25200-056
Sc26196	Santa Cruz Biotechnology	sc-361350
DMXAA	Invivogen	Tlrl-dmx
polyIC	Invivogen	tlrl-pic
Linoleic acid	Sigma	L1376
Linolenic acid	Sigma	L2376
Oleic acid	Sigma	O7501
DMSO	Sigma	D2650
FFA free BSA	Sigma	A7030
Red ANTI-FLAG M2 Affinity Gel	Sigma	F2426
3X Flag peptide	Sigma	F4799
HA probe	Santa Cruz Biotechnology	Sc-7392
HA peptide	Thermo Scientific	26184
Trizol	Thermofisher	15596018
super script IV	Thermofisher	18090050
TB Green Premix Ex Taq	TAKARA	RR420W
Rnase out	Thermofisher	10777019
dNTP mix 10mM	Thermofisher	18427013
Kit TURBO DNA-free	Thermofisher	AM1907
protein assay dye reageant	Biorad	5000006
TWEEN 80	Sigma	P4780-100ML
PEG300	Sigma	8074841000
Jet Prime tranfection kit	Ozyme	POL114-75
Accu-Chek PERFORMA	Roche	N/A
Rectal probe for mice	Kent Scientific	RET-3
Insulin	Sigma	I9278
Glucose	Sigma	G7021
Pyruvate	Sigma	P2256
Critical commercial assays
Mouse Insulin ELISA	ALPCO Diagnostics	80-INSMSU-E01
Silver Quest	Invitrogen	LC6070
Trans-Blot Turbo Transfer Pack 0.2 μm Nitrocellulose Midi	Biorad	1704159
Novex WedgeWell 10%, Tris-Glycine	Thermofisher	XP00105BOX
Novex WedgeWell 12%, Tris-Glycine	Thermofisher	XP00125BOX
SuperSignal West Pico PLUS Chemiluminescent Substrate	Thermofisher	34577
GenElute Total RNA Purification Kit	Sigma	RNB200
SuperSignal West Femto Maximum Sensitivity Substrate	Thermofisher	34094
Deposited data
MS/MS Data	This paper	https://doi.org/10.17632/23drspzh3s.1
Experimental models: Cell lines
MEF WT, Sting−/−& cGas−/−	Soren R. Paludan Lab at Aarhus University, Aarhus	N/A
MEF WT & Trex−/−	Jan Rehwinkel Lab at Oxford University, Oxford	N/A
Caco2	Clone TC7; provided by Nolwenn Jouvenet’s lab at Institut Pasteur, Paris	RRID:CVCL_0025
T98G	Caroline Goujon Lab at IRIM, Montpellier	RRID:CVCL_0556
THP1	ATCC	RRID:CVCL_0006
Vero	Raphael Gaudin, IRIM,Montpellier	RRID:CVCL_0059
293T	ATCC	RRID:CVCL_0063
Experimental models: Organisms/strains
Sting deficient mice	Lei Jin, Albany Mecical College, USA	Tmem173 < tm1Camb >
Transgenic FLP recombinase	PCEA, Montpellier, France	Tg(CAG-Flpo)1Afst
Transgenic LysM-Cre mice	Michael Hahne, IGMM,France	Lyz2tm1(cre)Ifo/J
Cgas deficient mice	EMMA consortium	Mb21d1tm1a(EUCOMM)Hmgu
Oligonucleotides
Primers for gene expression analysis, see Table S3	N/A	N/A
Guide RNAs for the CRISPR-Cas9 system, see Table S3	Gift from C. Goujon and B. Bonaventure, Montpellier, IRIM	N/A
Software and algorithms
Prism Software	GraphPad	Version 9.1
FlowJo software	N/A	10.7.1 version
ImageJ	N/A	N/A
CLAMS software	Oxymax	Columbus Instruments
Other
CHOW diet	Safe diet	A04
HFD 60% diet	Safe diet	292HF
OMEGA STD diet; See Table S2	Safe diet	Fat controlled custom diet for Mice U8978 v209
OMEGA3L diet; See Table S2	Safe diet	Fat controlled custom diet for Mice U8978 v211