Figure 4.

LUCAT1 overexpression decreases MSC apoptosis

(A) TUNEL staining with nuclei identified via DAPI staining (bar, 100 μm) of the control and LUCAT1-overexpressing MSCs under 500-μM H₂O₂ for 1 h. (B) Quantification of the TUNEL-positive nuclei (n = 3). (C) Annexin V-APC/PI staining was used to assess cell apoptosis and was detected by FACS. (D) The late (Q2) apoptotic rates were quantified (n = 3). (E) Cleaved caspase-3, Bax, and Bcl-2 levels were measured by western blots. (F) Quantitative analysis of Cleaved caspase-3, Bax, and Bcl-2 protein expression (n = 3). The data are presented as the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.