Figure 7.

LUCAT1 regulates FOXQ1 expression dependent on JMJD6

(A) Representative image of SDS-PAGE gels showing proteins pulled down by biotin-labeled LUCAT1 and LUCAT1 anti-sense antibodies (anti-sense). (B) Western blot analysis was used to detect JMJD6 expression in proteins retrieved from LUCAT1 and anti-sense pull-down assays. (C) RNA immunoprecipitation using an anti-JMJD6 antibody to assess endogenous JMJD6 binding with LUCAT1 in MSCs. Immunoglobulin G (IgG) was used as the control. LUCAT1 was detected by qRT-PCR products with specific primers at sites 2726–2886, and H₂O was used as the negative control. The results are shown by nucleic acid gel electrophoresis. (D and E) With JMJD6-knockdown treatment, the expression of Cleaved caspase-3 was assessed (n = 3). (F and G) With JMJD6-knockdown treatment, the expression of FOXQ1 was assessed (n = 3). All data are presented as the mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.