Figure 2.

Oocyte-specific knockout of Lonp1 compromises oocyte development in mice. (a) Photographs of 6-week-old Lonp1^loxp/loxp and Lonp1^Gdf9Cre cKO mice. Scale bar = 500μm. (b) Micrographs of HE-stained ovarian sections from 6-week-old Lonp1^loxp/loxp and Lonp1^Gdf9Cre cKO mice. The asterisks indicate oocytes. Scale bar = 50 μm. (c) Ovary index values of 6-week-old Lonp1^loxp/loxp and Lonp1^Gdf9Cre cKO mice (n=6, *p < 0.05, Student's t test). (d) Numbers of superovulated MII oocytes in 6-week-old Lonp1^loxp/loxp and Lonp1^Gdf9Cre cKO mice (n=6, ***p < 0.001, Student's t test). (e and f) Total follicle numbers and numbers of follicles in different developmental stages per ovary in 6-week-old Lonp1^loxp/loxp and Lonp1^Gdf9Cre cKO mice (n=6, *p < 0.05, ***p < 0.001, Student's t test and one-way ANOVA test). (g) Photographs of ovaries from 12-week-old Lonp1^loxp/loxp and Lonp1^Gdf9Cre cKO mice. Scale bar = 500 μm. (h) Micrographs of HE-stained ovarian sections from 12-week-old Lonp1^loxp/loxp and Lonp1^Gdf9Cre cKO mice. Scale bar = 500 μm. The asterisks indicate oocytes. (i) Ovary index values of 12-week-old Lonp1^loxp/loxp and Lonp1^Gdf9Cre cKO mice (at least 6 mice per group were included, ****p < 0.0001, Student's t test). (j) Serum concentrations of anti-Mullerian hormone (AMH) in 12-week-old Lonp1^loxp/loxp and Lonp1^Gdf9Cre cKO mice (n=6, **p < 0.01, Student's t test).