Figure 2.
Binding characteristics and transduction of SARS-CoV-2 VOCs
(A) BHK-21 cells stably expressing GFP and hACE2 were incubated with His-tagged RBDs from prototype SARS-CoV-2, VOCs, and pangolin-origin GD/1/2019. Allophycocyanin (APC)-conjugated anti-His antibodies were used to detect the His-tagged protein binding to the cells. Representative results from three experiments are shown. The frequency of RBD-binding positive cells in ACE2-GFP positive cells are labeled in the upright corner.
(B) Transduction efficiency of pseudotyped prototype SARS-CoV-2, D614G variant, and VOCs. The GFP-positive cells were quantified with CQ1 confocal image cytometer (Yokogawa), and representative results from three experiments are shown. Statistical significance was analyzed with a t test comparison between VOCs and D614G.
(C) SPR characterization of RBDs from prototype SARS-CoV-2, VOCs, and pangolin-origin GD/1/2019 interacting with hACE2. Dissociation constant (KD) indicates mean ± SD of three independent replicates. Actual and fitted curves are colored in black and red, respectively.
See also Figure S2.