Fig. 4.
Antiviral properties of lixiviates in contact with the different materials under study. A) 24 h after the incubation of 500 μl of the different materials with 1 ml of complete DMEM median, lixiviates were incubated with 106 VSV-GFP infective particles. TCID50/ml represents the viable virus remaining after 1 h of incubation with these conditionate media. B) 1/2, 1/5 and 1/10 serial dilutions of the lixiviate form samples showing some inhibitory activities were analyzed to determine their TCID50 against VSV-GFP. C) As in A) but virus was incubated with lixiviates for 24 h. D) Left: Graph represents the percentage of G3(▲), Cu(⧫), and Ag(•) samples that show antiviral ability versus their dilution value at 1 h of incubation. The 0.05% of the control is taken as the cutoff value, below which a sample is considered to have anti-viral activity. Right: projection of the inhibitory potential of each lixiviate. The fit to the experimental points was made based on a 4-parameter logistic model forcing the parameter corresponding to the Lower Value to zero. E) ICP analysis of elements present in lixiviates from A). Kluskal-Wallis test was used to determine statistical differences. ∗∗∗: p < 0.001; ∗∗: p < 0.01; ∗: p < 0.05; ns: not significate. N = 3. Error bars correspond to ±SD.