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. 2021 Dec 23;12:794496. doi: 10.3389/fendo.2021.794496

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Copyright © 2021 Jiang, Fei, Tong, Zhou, Zhu, Jin, Shi, Zhou, Ma, Yu, Yang and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Estrogen ameliorated ovarian fibrosis induced by Eggerthella lenta in mice. (A) Schematic diagram of the animal experimental protocol. (B) The expression of TGF-β1, α-SMA, and collagen I in mice ovarian tissues was detected using immunohistochemistry. Data are representative images obtained upon examination of mice in the control, E. lenta, and E. lenta + E2 group. (C) Staining intensity of TGF-β1, α-SMA, and collagen I expression analyzed via H-SCORE analysis and compared using one-way analysis of variance (ANOVA), followed by Newman-Keuls multiple comparison test using GraphPad Prism 7 (n = 6); scale bar = 100 μm; mean ± SEM, (*P < 0.05, **P < 0.01, NS, not significant).