Intramyocardial delivery of Anc80L65 resulted in increased number of viral genome copies and number of vector copies per cell in the heart compared to AAV9. Whereas, collateral organs expression was much lower in Anc80L65 compared to AAV9 (n=30). The level of GAPDH DNA was used to calculate normalized transfection. Data were reported as viral genome copies (gc) per 100 ng of DNA.
ns P > 0.05
* P ≤ 0.05
** P ≤ 0.01
*** P ≤ 0.001
5a. qPCR analysis of Anc80L65 versus AAV9 vector distribution in different organs.
5b. Average number of vector genome copies Anc80L65 versus AAV9 per cell in different organs.