FIG. 11.

Immunoblot analysis of GFP-Fes CC1+2 fusion proteins following gel filtration. Peak fractions from Sephacryl S-300 chromatography of the GFP-Fes CC1+2 fusion proteins shown in Fig. 10 were pooled, concentrated, and resolved by SDS-PAGE. The proteins were transferred to polyvinylidene difluoride membranes and probed with an antibody to GFP. Results are shown for wild-type GFP-Fes CC1+2 (WT), the CC1 mutation (L145P) protein, the CC2 mutation (L334P) protein, and the double mutant protein (2LP). The numbers above each lane refer to the major peaks shown in Fig. 10.