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Focus-forming activity of GFP-Fes proteins in Rat-2 fibroblasts. Rat-2 fibroblasts were infected with recombinant GFP-Fes retroviruses as described in Materials and Methods. For focus-forming assays, infected cells were plated in 60-mm-diameter dishes and incubated in the presence of G418 for 14 days. Scanned images of the stained cultures are shown, which appear as confluent monolayers with transformed foci (where present) growing on top.
