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. 2021 Dec 23;12:778828. doi: 10.3389/fmicb.2021.778828

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Copyright © 2021 Qi, Ma, Chang, Li, Ding and Yuan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic diagram of PET degradation by synthetic microbial consortia. (A) Bs_PETase (B1) and Bs_MHETase (B2) were engineered to secrete PETase and MHETase to degrade PET and a two-species microbial consortium was constructed. (B) Due to PETase inhibition by TPA, R. jostii (Rj) was added, resulting in a three-species microbial consortium. (C) To further utilize EG, P. putida (Pp) was introduced and a four-species microbial consortium was constructed.