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. 2021 Oct 26;220(1):iyab184. doi: 10.1093/genetics/iyab184

Figure 1.

Figure 1

The length scale of multivalent interactions is evolutionary conserved in fungi species Dcp2. (A) Architecture of Dcp2 in S. cerevisiae with the regulatory domain (in red), the NUDIX catalytic domain (in orange), and the disordered C-terminal domain (in white). Within the disordered C-terminal domain helical leucine-rich short linear motifs are responsible for the multivalency of Dcp2. (B) Multiple sequence alignment of the C-terminal domain of Dcp2 in 48 fungal species within the phylum of Ascomycota spanning ∼600 million years of evolution. HLMs, shown as blue columns, are highly conserved within the C-terminal domain of Dcp2. (C) The number of HLMs positively correlates with the length of the C-terminal domain of Dcp2 in fungi (Spearman correlation; R = 0.44, P = 0.0017). (D) The incidence of HMLs in biological sequences (shown in red) is ∼35 times higher than that of neutrally evolved sequences (shown in blue). (E) The distribution of spacer lengths (sequences that separate HLMs) in real Dcp2 sequences (shown in red), and in neutrally evolved sequences (shown in blue). We compared the two distributions and calculated the P-value for rejecting the null hypothesis that these distributions are indistinguishable by Kolmogorov–Smirnov (KS) test.