Figure 3.

PkLDH accumulation in P. knowlesi cultures. Percentage parasitemia in synchronized cultures was monitored using microscopy, and the PkLDH level in the iRBC pellet and supernatant from 1 mL of synchronized culture was quantified using PvLDH assay and in three replicates over 30 hours. For estimating parasitemia, one of three replicates was examined. Time zero represents time of synchronization. Error bars indicate standard deviation of the mean. This figure appears in color at www.ajtmh.org.