FIG. 7.
RIP140 represses estrogen receptor-dependent transcription. The ability of full-length RIP140, wild type and mutants, to repress transcription of an (ERE)2-pS2-CAT reporter gene was analyzed in HepG2 cells treated with 10 nM estradiol. Cells were transfected with 100 ng of CAT reporter, 20 ng of SV40-lacZ, and 100 ng of pEFRIP140 wild type and mutants in the absence or presence of pCMV-hCtBP1. The total concentration of DNA was kept constant by addition of empty pcDNA3 vector. CAT protein production was measured 48 h after transfection, and values are normalized to β-galactosidase activity.