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PIP2 levels are reduced with age in SHRs in comparison to control WKY rats. (A) Expression of PIP2 from left ventricular tissue in two different age group of animals with young animals < 10 months of age (WKY n = 8 rats; SHR n = 6 rats) and old animals > 15 months (WKY n = 8 rats; SHR n = 9 rats). (B) Graph of relative protein expression normalized to GAPDH. Values indicated mean ± SEM here and in all subsequent experiments. Comparisons were made using an unpaired t-test. *p < 0.05.
