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. 2021 Dec 23;12:782767. doi: 10.3389/fphys.2021.782767

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Copyright © 2021 Zhou, Singh, Monnier, Marszalec, Gao, Jin, Frisk, Louch, Verma, Krishnamurthy, Nico, Mulla, Aistrup, Kishore and Wasserstrom.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PIP2 depleting agents adversely affected organizational index (OI) of TTs in isolated adult rat ventricular myocytes. (A–D) 2-D images of control (left panels) and treated (right panels) myocytes. (E) Summary of OI changes untreated cells (35 myocytes in 3 rats) and after treatment with wortmannin (15 μmol/L, 2 h, n = 35/3), m-3M3FBS (30μmol/L, 3 h, n = 37/5), and Et-1(400 nmol/L) + m-3M3FBS (30 μmol/L, 45 min, n = 35/4) and Et-1 (400 nmol/L, 6 h, n = 54/4). *p < 0.05 using Dunn’s test following a one-way ANOVA performed on all cells. Pixel size was 0.45 μm with the pinhole adjusted to Airey units = 1.