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. 2021 Dec 23;12:782767. doi: 10.3389/fphys.2021.782767

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Copyright © 2021 Zhou, Singh, Monnier, Marszalec, Gao, Jin, Frisk, Louch, Verma, Krishnamurthy, Nico, Mulla, Aistrup, Kishore and Wasserstrom.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Calcium transients in isolated adult ventricular myocytes after PIP2 depletion. (A–D) 2-D images with associated linescan images from treated and non-treated myocytes. Pacing was performed at basic cycle lengths of 2,000 and 1,000 ms. Arrows shows areas of delayed Ca²⁺ release along the cell length in the myocytes. (E–G) Shows summaries of rise time, time to peak and decay time from control and treated cells at a pacing cycle length of 1,000 ms. Control (n = 18–28 myocytes from 6 rats), wortmannin (27–36/5), m-3M3FBS (27/3), Et-1+m-3M3FBS (n = 22–24/3). *p < 0.05 using a Dunn’s test following a one-way ANOVA performed on cells. Pixel size was 0.2 μm with the pinhole adjusted to Airey units = 1.