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. 2021 Dec 23;12:801795. doi: 10.3389/fmicb.2021.801795

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Copyright © 2021 Shehata, Kiefer, Morovic and Newmaster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Multiple sequence alignment from NCBI multiple sequence alignment viewer 1.20.1. The amplicon sequence of (A) Bifidobacterium animalis subsp. lactis Bi-07 and (B) B. animalis subsp. lactis DSM 15954 assays were searched on GenBank using the blastn function against the Nucleotide collection database to find matches in all publicly available genome sequences. The probe sequence is in a blue box and the locked nucleic acid bases are in an orange box. The single nucleotide polymorphism (SNP) identified in Bi-07 was unique to strain Bi-07 compared to all other Bifidobacterium animalis subsp. lactis strains deposited in GenBank, while the SNP identified in DSM 15954 was unique to all strains except IDCC4301, BF052, RH, and i797, as of August 2021.