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. 2021 Dec 23;12:801795. doi: 10.3389/fmicb.2021.801795

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Copyright © 2021 Shehata, Kiefer, Morovic and Newmaster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Evaluating the analytical sensitivity and efficiency of the developed strain-specific assays. (A) Bifidobacterium animalis subsp. lactis DSM 15954 assay and (B) Bifidobacterium animalis subsp. lactis Bi-07 assay. Three 10-fold dilution series were prepared from three starting DNA concentrations (10 ng/μl, 5 ng/μl, and 2 ng/μl). Each dilution series was prepared at five dilution points and each dilution was tested in triplicate. Limits of detection were 5 pg, corresponding to 2388 target copies, and 0.5 pg, corresponding to 239 target copies, for DSM 15954 and Bi-07 assays, respectively.