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Evaluating repeatability and reproducibility of the developed strain-specific assays. (A) Bifidobacterium animalis subsp. lactis DSM 15954 assay and (B) Bifidobacterium animalis subsp. lactis Bi-07 assay. Five samples at three different DNA concentrations (1 ng/μl, 0.1 ng/μl and 0.01 ng/μl) were used. Each assay was repeated on a different day to evaluate repeatability and was repeated on a different real-time PCR platform (bCUBE and QuantStudio 5) to evaluate reproducibility.
