FIG. 3.

Inactivation of AURA significantly delays cell cycle progression in germinating spores. Spores with or without AURA function were germinated on coverslips in the presence of 5 μg of nocodazole per ml, fixed, and stained with DAPI. The CMI was determined as the percentage of cells with condensed DNA. Shown are CMIs of wt and aurA3Δ alcA::aurA spores germinated in medium containing glycerol as the sole carbon source (A), of wt and aurA3Δ alcA::aurA spores germinated in medium containing glucose (B), and of wt spores germinated in the presence or absence of AbA (C).