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. 2021 Dec 23;12:796049. doi: 10.3389/fmicb.2021.796049

FIGURE 5.

FIGURE 5

Roles of WSV056, LvSpr, and LvNos to regulating host innate immunity by WSSV. (A,B) NO production (A) and WSSV load (B) of single gene knockdown of LvSpr, LvNos, and WSV056 and double gene knockdown of WSV056 + LvSpr and WSV056 + LvNos. The dsRNAs were injected into shrimp twice in a 48 h interval. From 24 h after the first injection, shrimp started to be fed with BH4 or DETA/NO supplemented diet. Shrimp hemocytes were sampled at 48 h after the second injection of dsRNA and WSSV virions. Different letters (above each bar) indicate a significant difference (P < 0.05) in the mean NO production (A) or WSSV load (B) among the samples from different groups. (C) qRT-PCR analysis of the RNA interference efficiency of WSV056 in the group with single gene knockdown of WSV056 and double gene knockdown groups of WSV056 + LvSpr and WSV056 + LvNos infected with WSSV. Expression values were normalized to those of β-actin.